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Dimethyl Sulfoxide Perturbs Cell Cycle Progression and

DMSO (Sigma, . no. D2650) suitable for cell culture was used in the present study. Collection and in vitro maturation (IVM) of pig cumulus-oocyte complexes (COCs) Porcine ovaries were collected from a local slaughterhouse and transported to the laboratory while maintained at 30–35C.

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REAGENTS AND MEDIUM PREPARATION

Dimetyl Sulphoxide (DMSO) Sigma # D2650 Medium preparation, 10ml: 10ml Volume MEF medium 6ml FBS 2ml DMSO 2ml Add MEF medium and FBS to a 10ml Falcon tube. Filter sterilize at 0.22 m. Add DMSO. Store at +4 C. FREEZING MEDIUM I and II FOR HPSC I Freezing medium components: Stock conc. Supplier . number

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The cytotoxic effects of dimethyl sulfoxide in mouse

DMSO (Sigma-Aldrich, D2650) was added to KSOM at concentrations of 0%, 0.5%, 1%, and 2% (v/v). Osmolarity levels in each group were determined using the Advanced Model 3320 Micro-Osmometer (Advanced Instrument, 3320) according to the manufacturer's instructions.

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Dimethyl Sulfoxide Perturbs Cell Cycle Progression and

DMSO (Sigma, . no. D2650) suitable for cell culture was used in the present study. Collection and in vitro maturation (IVM) of pig cumulus-oocyte complexes (COCs) Porcine ovaries were collected from a local slaughterhouse and transported to the laboratory while maintained at 30–35C.

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Safety Data Sheet

Sigma - D2650 Page 2 of 7 Eyes May cause eye irritation. Ingestion May be harmful if swallowed. Aggravated Medical Condition Avoid contact with DMSO solutions containing toxic materials or materials with unknown toxicological properties. Dimethyl sulfoxide is readily absorbed through skin and may carry such materials into the body., 3.

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Dimethyl Sulfoxide, Hybri

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury.

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REAGENTS AND MEDIUM PREPARATION

Dimetyl Sulphoxide (DMSO) Sigma # D2650 Medium preparation, 10ml: 10ml Volume MEF medium 6ml FBS 2ml DMSO 2ml Add MEF medium and FBS to a 10ml Falcon tube. Filter sterilize at 0.22 m. Add DMSO. Store at +4 C. FREEZING MEDIUM I and II FOR HPSC I Freezing medium components: Stock conc. Supplier . number

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Electrophysiology

Electrophysiology › DMSO (dimethyl sulfoxide) (Sigma, . no. D2650) Neurotransmitters or ligands (e.g., acetylcholine, glutamate) 44 μL of DMSO to one vial of fluo-4 AM (50 μg) and vortex thoroughly. You may store the fluo-4 AM reconstituted in DMSO protected from light, frozen, and desiccated for

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Chromatin Immunoprecipitation (ChIP) Assay Protocol

Weigh 1.742g PMSF (Sigma-Aldrich, catalog number 10837091001) and add DMSO (Sigma-Aldrich, catalog number D2650) to a final volume of 100mL. Dissolve completely. PBS: HyClone™ Phosphate Buffered Saline (1X) (catalog number SH30256.01) or equivalent product from other vendor.

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Freezing and thawing of cell lines. EuroMAbNet

Gently resuspend the cell pellet in 1.5ml of freezing medium containing 10% Dimethylsulphoxide (DMSO, from Sigma D2650) in 90 % FCS previously heat-inactivated). Transfer 1.5ml of the resulting cell suspension into a freezing vial (Thermo 375418). Seal the vial (finger tight) to

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Dimethyl sulfoxide

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury.

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In vitro expansion and genetic modification of

Organoids can be frozen in a standard cell freezing media containing 10% dimethyl sulfoxide (DMSO). For use in functional assays, organoids were trypsinized with vigorous pipetting which dissociated cells into homogeneous small aggregates.

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【】DMSO

Jan 28, 2020Dimethyl sulfoxide (≥99.5%, DMSO) 67-68-5: 20L: 6,800.00: Sigma-D5879: : () Dimethyl sulfoxide (≥99.5%,cell culture tested) 67-68-5: 100ML: 235.00: Sigma-D4540: : () Dimethyl sulfoxide (≥99.5%,cell culture tested) 67-68-5: 500ML: 574.00: Sigma-D4540: : (Hybri-Max)

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Dimethyl sulfoxide

Edit article at Wikipedia Dimethyl sulfoxide ( DMSO) is an organosulfur compound with the formula ( CH3) 2SO. This colorless liquid is an important polar aprotic solvent that dissolves both polar and nonpolar compounds and is miscible in a wide range of organic solvents as well as water. It has a relatively high melting point.

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Dimethyl Sulfoxide (DMSO)

Dimethyl Sulfoxide (DMSO) sc-202581 Hazard Alert Code Key: EXTREME HIGH MODERATE LOW Section 1 - CHEMICAL PRODUCT AND COMPANY IDENTIFICATION PRODUCT NAME Dimethyl Sulfoxide (DMSO) STATEMENT OF HAZARDOUS NATURE CONSIDERED A HAZARDOUS SUBSTANCE ACCORDING TO OSHA 29 CFR 1910.1200. NFPA SUPPLIER Company: Santa Cruz

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Transient Treatment of Human Pluripotent Stem Cells with

To investigate the effect of DMSO on latter stages of a differentiation protocol, HUES8 hESCs were pretreated for 24 h with 2% DMSO prior to differentiation to β cells following a 20 day directed differentiation protocol described in Figure 4a 35.

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SIGMA

Sigma - D2650 Page 2 of 7 Eyes May cause eye irritation. Ingestion May be harmful if swallowed. Aggravated Medical Condition Avoid contact with DMSO solutions containing toxic materials or materials with unknown toxicological properties. Dimethyl sulfoxide is readily absorbed through skin and may carry such materials into the body., 3.

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Dimethyl sulfoxide ≥99.7%, Hybri

Synonyms: Methyl sulfoxide, DMSO, Methyl sulphoxide. D2650-5X5ML D2650-5X10ML D2650-100ML. 220121-737EA 82.75 USD InStock. 220121-737 220121-736 220121-735. Dimethyl sulfoxide ≥99.7%, Hybri-Max™, Sterile-Filtered. Dimethyl sulphoxide. Hybri-Max™, sterile-filtered, BioReagent, suitable for

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Propagation and Maintenance of Mouse N2a Cells

Serum in medium will inhibit trypsin from further digestion. 4. Transfer 1 ml to a fresh TC flask containing 9 ml growth medium (for a 1:10 split; or 250 ul into 9.75 ml medium for a 1:40 split). Transfection: At JHMI: Approximately 5 x 105 cells plated into 60mm petri dishes will

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(DMSO),SIGMAD5879/D4540/D2650

Dimethyl sulfoxide (≥99.5%, DMSO) 67-68-5: 20L: 6,800.00: Sigma-D5879: : () Dimethyl sulfoxide (≥99.5%,cell culture tested) 67-68-5: 100ML: 235.00: Sigma-D4540: : () Dimethyl sulfoxide (≥99.5%,cell culture tested) 67-68-5: 500ML: 574.00: Sigma-D4540: : (Hybri-Max)

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